Western blot analysis of SEMA4C in extracts of various cell lines. Samples were incubated with SEMA4C Polyclonal antibody (Product # PA5-96975) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry analysis of SEMA4C in paraffin-embedded human lung cancer using SEMA4C Polyclonal Antibody (Product # PA5-96975) at a dilution of 1:200.
Supplier Page from Thermo Fisher Scientific for SEMA4C Antibody